Microplate Readers and Reader Accessories

Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.

The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.

In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.

Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.

Features may include:

Monochromator- or filter-based wavelength selection
Visible only or UV/Vis spectrum, fluorescence, and luminescence
Multiple photomultiplier tubes (PMTs) for simultaneous assays
Temperature and CO₂/O₂controls
Modular design for adding functionality

Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.

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886

Revvity Health Sciences Inc HTRF Anti histone H3K4 Me2-EuK mAb conjugate, 10,000 Assay Points

HTRF Anti histone H3K4 Me2-EuK mAb conjugate, 10,000 Assay Points

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887

THERMO SCI LSG 3P EMISSION FILTER 520 NM HBW10

EMISSION FILTER 520 NM HBW10

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888

Revvity Health Sciences Inc HTRF Human & Mouse Ataxin 2 Detection Kit, 100,000 Assay Points

HTRF Human & Mouse Ataxin 2 Detection Kit, 100,000 Assay Points

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889

Revvity Health Sciences Inc HTRF Human Total SMARCA-2 Detection Kit, 500 Assay Points

HTRF Human Total SMARCA-2 Detection Kit, 500 Assay Points

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890

Revvity Health Sciences Inc HTRF AAV5 Detection Kit Standard

HTRF AAV5 Detection Kit Standard

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891

Molecular Devices Filter Slide, Empty, Excitation, 6-Filter Capacity

Empty excitation filter slide for FilterMax multi-mode microplate readers. Supplied unpopulated to allow custom installation of excitation filters for fluorescence and many luminescence methods. The slide installs into the instrument filter track and secures filters with small hex screws.

Supplied empty for custom filter configurations.
Accepts up to six individual filters per slide.
Distinct sizes for excitation and emission slides to prevent misinstallation.
Install and handle by the tab to avoid contaminating filter surfaces.
When configured as an excitation slide, not compatible with fluorescence polarization (FP) methods.